Ohshima K, Takeo H, Kikuchi M, Kozuru M, Uike N, Masuda Y, Yoneda S, Takeshita M, Shibata T, Akamatsu M
Department of Pathology, School of Medicine, Fukuoka University, Japan.
Histopathology. 1994 Dec;25(6):569-79. doi: 10.1111/j.1365-2559.1994.tb01376.x.
To investigate the relationship of Epstein-Barr virus (EBV) and angioimmunoblastic lymphadenopathy with dysproteinemia, we performed DNA analysis using the polymerase chain reaction (PCR), Southern blot, in situ hybridization, and immunohistochemical analysis of lymph nodes in five patients who were followed up and biopsied more than once. In the course of the disease, nodal architecture diminished, cellular atypia worsened, and clear cells increased in number. In the DNA analysis of the receptor genes, the clonal population increased in number. EBV nucleic acid sequences were found by either PCR or in situ hybridization in all examined nodes. The number of EBV-positive cells varied widely among the cases and throughout the course of the disease in the same patients. The analysis of EBV terminal repeats or lymphocyte-determined membrane antigen genes showed polyclonal populations of EB-infected cells. EBV-positive cells possessed intermediate- to large-sized nuclei, and the cells with large nuclei, especially, expressed latent membrane protein of EBV. These large cells varied among the cases. Double-labelling immunohistochemistry/in situ hybridization studies demonstrated that most of the EBV-positive cells expressed B-cell antigen (CD20). The presence of EBV seems to be associated with the selective defects of the immune system, rather than with the direct pathogenesis of angioimmunoblastic lymphadenopathy.
为研究爱泼斯坦-巴尔病毒（EBV）与血管免疫母细胞性淋巴结病伴蛋白异常血症之间的关系，我们对5例接受多次随访及活检的患者的淋巴结进行了DNA分析，采用聚合酶链反应（PCR）、Southern印迹法、原位杂交和免疫组织化学分析。在疾病过程中，淋巴结结构破坏，细胞异型性加重，透明细胞数量增加。在受体基因的DNA分析中，克隆群体数量增加。在所有检测的淋巴结中，通过PCR或原位杂交均发现了EBV核酸序列。EBV阳性细胞的数量在不同病例以及同一患者疾病的整个过程中差异很大。对EBV末端重复序列或淋巴细胞决定的膜抗原基因的分析显示，EB感染细胞为多克隆群体。EBV阳性细胞具有中等大小到大型的细胞核，尤其是那些具有大细胞核的细胞，表达EBV的潜伏膜蛋白。这些大细胞在不同病例中有所不同。免疫组织化学/原位杂交双标记研究表明，大多数EBV阳性细胞表达B细胞抗原（CD20）。EBV的存在似乎与免疫系统的选择性缺陷有关，而不是与血管免疫母细胞性淋巴结病的直接发病机制有关。
