伴T细胞淋巴瘤的血管免疫母细胞性淋巴结病中EB病毒DNA及EB病毒决定的核抗原的检测

Detection of Epstein-Barr virus DNA and EBV-determined nuclear antigen in angioimmunoblastic lymphadenopathy with dysproteinemia type T cell lymphoma.

作者信息

Kon S, Sato T, Onodera K, Satoh M, Kikuchi K, Imai S, Osato T

机构信息

Department of Pathology, Sapporo Medical University, Japan.

出版信息

Pathol Res Pract. 1993 Dec;189(10):1137-44. doi: 10.1016/S0344-0338(11)80835-6.

DOI:10.1016/S0344-0338(11)80835-6

PMID:8183733

Abstract

Six cases of angioimmunoblastic lymphadenopathy with dysproteinemia (AILD)-like T cell lymphoma were analyzed by immunohistochemical staining, polymerase chain reaction (PCR) and Southern blot analysis. Five cases out of six showed gene rearrangements of the T cell receptor beta chain, indicating the existence of a clonal T cell proliferation. Epstein-Barr virus (EBV) DNA was detected in all six AILD type cases by PCR amplifying the sequence located in the internal repeat I, and confirmed by Southern blot hybridization, using a BamHI-W fragment as a probe. Detection of the EBV genome occurred more frequently as compared with other types of lymphoid disorders. Furthermore, EBV determined nuclear antigen (EBNA) was detected in UCHL-1 (CD45RO, pan-T cell marker) positive cells by immunostaining. These results suggest that a significant number of AILD type cases are T cell origin lymphomas and EBV infection may be relevant to the biological features of this type of lymphoma.

摘要

通过免疫组织化学染色、聚合酶链反应（PCR）和Southern印迹分析，对6例血管免疫母细胞性淋巴结病伴蛋白血症（AILD）样T细胞淋巴瘤进行了分析。6例中有5例显示T细胞受体β链基因重排，表明存在克隆性T细胞增殖。通过PCR扩增位于内部重复序列I中的序列，在所有6例AILD型病例中均检测到爱泼斯坦-巴尔病毒（EBV）DNA，并使用BamHI-W片段作为探针通过Southern印迹杂交进行了证实。与其他类型的淋巴系统疾病相比，EBV基因组的检测更为频繁。此外，通过免疫染色在UCHL-1（CD45RO，全T细胞标志物）阳性细胞中检测到EBV核抗原（EBNA）。这些结果表明，相当数量的AILD型病例是T细胞起源的淋巴瘤，EBV感染可能与这类淋巴瘤的生物学特征相关。