正常T淋巴细胞可表达两种不同的T细胞受体β链：对等位基因排斥机制的启示。
Normal T lymphocytes can express two different T cell receptor beta chains: implications for the mechanism of allelic exclusion.
作者信息
Padovan E, Giachino C, Cella M, Valitutti S, Acuto O, Lanzavecchia A
机构信息
Basel Institute for Immunology, Switzerland.
出版信息
J Exp Med. 1995 Apr 1;181(4):1587-91. doi: 10.1084/jem.181.4.1587.
Abstract
We have examined the extent of allelic exclusion at the T cell receptor (TCR) beta locus using monoclonal antibodies specific for V beta products. A small proportion (approximately 1%) of human peripheral blood T cells express two V beta as determined by flow cytometric analysis, isolation of representative clones, and sequencing of the corresponding V beta chains. Dual beta T cells are present in both the CD45R0+ and CD45R0- subset. These results indicate that dual beta expression is compatible with both central and peripheral selection. They also suggest that the substantial degree of TCR beta allelic exclusion is dependent only on asynchronous rearrangements at the beta locus, whereas the role of the pre-TCR is limited to signaling the presence of at least one functional beta protein.
摘要
我们使用针对Vβ产物的单克隆抗体，研究了T细胞受体（TCR）β基因座上等位基因排斥的程度。通过流式细胞术分析、代表性克隆的分离以及相应Vβ链的测序确定，一小部分（约1%）人外周血T细胞表达两种Vβ。双β T细胞存在于CD45R0+和CD45R0-亚群中。这些结果表明，双β表达与中枢和外周选择均兼容。它们还表明，TCRβ等位基因排斥的显著程度仅取决于β基因座上的异步重排，而前TCR的作用仅限于发出至少一种功能性β蛋白存在的信号。
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