Lichtiger B, Trujillo J M, Burk K H, Drewinko B
Cancer. 1976 Apr;37(4):1788-99. doi: 10.1002/1097-0142(197604)37:4<1788::aid-cncr2820370425>3.0.co;2-v.
The present investigation was designed to demonstrate and identify the subcellular localization of sarcoma-associated antigen(s) (TAA) in an established human neurogenic sarcoma line (T cell line). Indirect immunofluorescence was used to screen sera from patients with sarcoma, nonsarcoma neoplasias, and from presumably normal blood bank donors. The positive sera were submitted to a series of absorptions to remove possible nonspecific cross-reacting antibodies. The target cells were then studied with electron microscopy, utilizing the indirect immunoperoxidase technique to determine the subcellular localization of the sarcoma-associated antigen(s). Findings indicate that sarcoma-bearing patients have circulating antisarcoma antibodies directed to antigen(s) localized at the surface membrane of T cells. These antibodies pertain to the IgM or IgG immunoglobulin class. The exact nature of the sarcoma-associated antigen(s) identified in this study is not known at the present time. However, the results obtained strongly suggest that these substances may represent a new type of tumor-associated antigen(s).
本研究旨在证明并确定肉瘤相关抗原（TAA）在已建立的人神经源性肉瘤细胞系（T细胞系）中的亚细胞定位。采用间接免疫荧光法筛选肉瘤患者、非肉瘤性肿瘤患者以及推测正常的血库供血者的血清。对阳性血清进行一系列吸收处理，以去除可能的非特异性交叉反应抗体。然后利用间接免疫过氧化物酶技术，通过电子显微镜研究靶细胞，以确定肉瘤相关抗原的亚细胞定位。研究结果表明，患肉瘤的患者体内存在循环抗肉瘤抗体，这些抗体针对位于T细胞表面膜的抗原。这些抗体属于IgM或IgG免疫球蛋白类别。目前尚不清楚本研究中鉴定出的肉瘤相关抗原的确切性质。然而，所获得的结果强烈表明，这些物质可能代表一种新型的肿瘤相关抗原。
