胶质瘤细胞系和标本上的细胞因子基因表达。
Cytokine gene expression on glioma cell lines and specimens.
作者信息
Yamanaka R, Tanaka R, Saitoh T, Okoshi S
机构信息
Department of Neurosurgery, Niigata University, Japan.
出版信息
J Neurooncol. 1994;21(3):243-7. doi: 10.1007/BF01063773.
Expression of the cytokine genes in human glioma cell lines and specimens was studied. Primers for 7 different human cytokine, IL-1 beta, IL-6, IL-8, GM-CSF, TGF-beta 1, TNF-alpha and IFN-gamma were used to analyze messenger RNA transcripts by polymerase chain reaction (PCR). Messenger RNA encoding for IL-1 beta, IL-6, IL-8, GM-CSF and TGF-beta 1 was found to be expressed in some of glioma cell lines. And those showed a proliferative response to IL-1 beta. IL-8 mRNA was found in 2 of 5 low grade gliomas, in 8 of 9 high grade gliomas. TGF-beta 1 mRNA was found in all gliomas, in 1 of 2 normal brains. IL-1 beta mRNA was only found in normal brains. TNF-alpha and IFN-gamma were not found in glioma cell lines and specimens. IL-8 mRNA was apt to be found more frequently among high grade glioma specimens.
对人胶质瘤细胞系和标本中细胞因子基因的表达进行了研究。使用针对7种不同人类细胞因子（白细胞介素-1β、白细胞介素-6、白细胞介素-8、粒细胞-巨噬细胞集落刺激因子、转化生长因子-β1、肿瘤坏死因子-α和干扰素-γ）的引物，通过聚合酶链反应（PCR）分析信使核糖核酸转录本。发现编码白细胞介素-1β、白细胞介素-6、白细胞介素-8、粒细胞-巨噬细胞集落刺激因子和转化生长因子-β1的信使核糖核酸在一些胶质瘤细胞系中表达。并且那些细胞系对白细胞介素-1β表现出增殖反应。在5例低级别胶质瘤中有2例发现白细胞介素-8信使核糖核酸，在9例高级别胶质瘤中有8例发现。在所有胶质瘤以及2例正常脑组织中的1例中发现转化生长因子-β1信使核糖核酸。白细胞介素-1β信使核糖核酸仅在正常脑组织中发现。在胶质瘤细胞系和标本中未发现肿瘤坏死因子-α和干扰素-γ。白细胞介素-8信使核糖核酸在高级别胶质瘤标本中更易频繁发现。
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