Mihara K, Harada H, Kawano M M
Nihon Rinsho. 1995 Mar;53(3):574-9.
Recent 2-color phenotypic analysis using anti-CD38 antibody reveals that plasma cells alone locate at CD38strong positive (CD38++) fraction and expression of adhesion molecules such as VLA-5 and MPC-1 can define VLA-5-MPC-1- immature, VLA-5-MPC-1+ intermediate and VLA-5+ MPC-1+ mature plasma (myeloma) cells. Furthermore, phenotypic analysis of plasma cells with anti-CD19 and -CD56 antibodies can distinguish normal (polyclonal) plasma cells from malignant (monoclonal) plasma cells; normal plasma cells from various tissues are all CD19+ CD56-, while malignant plasma cells are mostly CD19- CD56+. Therefore, this 2-color phenotypic analysis is very useful for differential diagnosis of bone marrow plasmacytosis, that is, myeloma, benign monoclonal gammopathy or polyclonal gammopathy, and furthermore contributes to understanding of differentiated stages of myeloma cells (immature, intermediate or mature myeloma cells).
近期使用抗CD38抗体进行的双色表型分析显示，仅浆细胞位于CD38强阳性（CD38++）部分，而诸如VLA-5和MPC-1等黏附分子的表达可界定VLA-5-MPC-1-未成熟、VLA-5-MPC-1+中间型以及VLA-5+ MPC-1+成熟浆（骨髓瘤）细胞。此外，用抗CD19和抗CD56抗体对浆细胞进行表型分析可区分正常（多克隆）浆细胞与恶性（单克隆）浆细胞；来自各种组织的正常浆细胞均为CD19+ CD56-，而恶性浆细胞大多为CD19- CD56+。因此，这种双色表型分析对于骨髓浆细胞增多症（即骨髓瘤、良性单克隆丙种球蛋白病或多克隆丙种球蛋白病）的鉴别诊断非常有用，而且有助于了解骨髓瘤细胞的分化阶段（未成熟、中间型或成熟骨髓瘤细胞）。
