Hermanns J, Debets F, Hoekstra R, Osiewacz H D
German Cancer Research Center, Department of Molecular Biology of Aging Processes, Heidelberg.
Mol Gen Genet. 1995 Mar 10;246(5):638-47. doi: 10.1007/BF00298971.
Three recently isolated wild-type strains of the ascomycete Podospora anserina were analyzed for the presence of linear mitochondrial plasmids. In one of these strains, designated Wat, at least 12 distinct plasmid-like elements were identified. From molecular analyses a minimum number of 78 individual linear molecules with proteins bound to their 5' ends was estimated. In addition, the different members of this family of typical linear plasmids were shown to possess a common central region and terminal sequences which differ from one plasmid to another due to the presence of different numbers of a 2.4 kb sequence module. Finally, the pWa6 plasmids share a high degree of sequence similarity with pAL2-1, a linear plasmid previously identified in mitochondria of a long-lived mutant of P.anserina. A mechanism is proposed which explains the generation of these distinct, closely related extra-chromosomal genetic traits.
对最近分离出的三种野生型子囊菌Podospora anserina菌株进行了线性线粒体质粒存在情况的分析。在其中一个命名为Wat的菌株中，至少鉴定出了12种不同的类质粒元件。通过分子分析估计，至少有78个单独的线性分子，其5'端结合有蛋白质。此外，该典型线性质粒家族的不同成员显示具有一个共同的中心区域和末端序列，由于存在不同数量的2.4 kb序列模块，不同质粒的末端序列有所不同。最后，pWa6质粒与pAL2-1具有高度的序列相似性，pAL2-1是先前在长寿突变型P.anserina线粒体中鉴定出的一种线性质粒。本文提出了一种机制，解释了这些不同的、密切相关的染色体外遗传特征的产生。
