Cummings D J, McNally K L, Domenico J M, Matsuura E T
Department of Microbiology/Immunology, University of Colorado School of Medicine, Denver 80262.
Curr Genet. 1990 May;17(5):375-402. doi: 10.1007/BF00334517.
The complete 94,192 bp sequence of the mitochondrial genome from race s of Podospora anserina is presented (1 kb = 10(3) base pairs). Three regions unique to race A are also presented bringing the size of this genome to 100,314 bp. Race s contains 31 group I introns (33 in race A) and 2 group II introns (3 in race A). Analysis shows that the group I introns can be categorized according to families both with regard to secondary structure and their open reading frames. All identified genes are transcribed from the same strand. Except for the lack of ATPase 9, the Podospora genome contains the same genes as its fungal counterparts, N. crassa and A. nidulans. About 20% of the genome has not yet been identified. DNA sequence studies of several excision-amplification plasmids demonstrate a common feature to be the presence of short repeated sequences at both termini with a prevalence of GGCGCAAGCTC.
本文给出了嗜鸟粪盘菌(Podospora anserina)菌株线粒体基因组的完整94,192 bp序列(1 kb = 10³ 个碱基对)。还给出了A菌株特有的三个区域,使该基因组大小达到100,314 bp。菌株s含有31个I类内含子(A菌株有33个)和2个II类内含子(A菌株有3个)。分析表明,I类内含子可根据二级结构及其开放阅读框归为不同家族。所有已鉴定的基因都从同一条链转录。除了缺少ATPase 9外,嗜鸟粪盘菌基因组所含基因与其他真菌如粗糙脉孢菌(Neurospora crassa)和构巢曲霉(Aspergillus nidulans)相同。约20%的基因组尚未鉴定。对几种切除 - 扩增质粒的DNA序列研究表明,其共同特征是两端存在短重复序列,其中GGCGCAAGCTC最为常见。