采用巢式聚合酶链反应法检测血清中的乙型肝炎病毒。
Detection of serum hepatitis B virus using a nested polymerase chain reaction assay.
作者信息
Lucotte G, Galzot P, Lu C Y, Bathelier C, Champenois T
机构信息
International Institute of Anthropology, Paris, France.
出版信息
Mol Cell Probes. 1994 Dec;8(6):437-40. doi: 10.1006/mcpr.1994.1062.
We described a nested polymerase chain reaction (PCR) assay to detect serum hepatitis B virus (HBV) DNA in patients. We compared the sensitivity of the PCR assay to that of slot-blot hybridization for detecting HBV DNA in serum and found that analysis by PCR provides a > 10(4) fold increase over the slot-blot assay. Most of the HBe Ag-positive patients were positive in PCR, and most of those being anti-HBe-positive were negative with this PCR assay.
我们描述了一种用于检测患者血清中乙型肝炎病毒（HBV）DNA的巢式聚合酶链反应（PCR）检测方法。我们比较了PCR检测方法与斑点杂交法检测血清中HBV DNA的灵敏度，发现PCR分析比斑点杂交法的灵敏度提高了10⁴倍以上。大多数HBeAg阳性患者的PCR检测结果为阳性，而大多数抗HBe阳性患者的PCR检测结果为阴性。
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