Seelig R, Ehrfeld H, Bottner C, Renz M, Seelig H P
Privates Institut für Immunologie und Molekulargenetik GmbH, Karlsruhe.
Dtsch Med Wochenschr. 1990 Aug 31;115(35):1307-12. doi: 10.1055/s-2008-1065158.
Results with the polymerase chain reaction and conventional DNA hybridizing technique (dot-blot) for the detection of hepatitis B virus (HBV) DNA were compared for 439 patients. In 261 patients who were positive for HBs antigen (Ag), HBV DNA was demonstrated with the polymerase reaction in all of the 69 HBe-Ag positive sera (dot-blot 56%), as well as in 81 (62%) of 131 anti-HBe positive sera (dot-blot 5%), in 29 (48%) of 61 HBe marker negatives (dot-blot 3%) and in six (29%) of 21 delta positive sera. Among HBs-Ag negative patients, HBV DNA was detected in six (22%) of 27 anti-HBc positive sera, but not in sera (n = 50) which were both anti-HBc and anti-HBs positive, as well as in HBV marker negative patients with liver disease (n = 30) and healthy controls (n = 50). If infectiousness is to be checked or in case of double infection, the more sensitive polymerase chain reaction is to be recommended for the detection of HBV DNA. HBe-Ag positive patients must be considered as infectious: tests for HBV DNA can in general be omitted.
对439例患者采用聚合酶链反应和传统DNA杂交技术（斑点印迹法）检测乙型肝炎病毒（HBV）DNA的结果进行了比较。在261例HBs抗原（Ag）阳性患者中，聚合酶反应在所有69例HBe-Ag阳性血清中均检测到HBV DNA（斑点印迹法为56%），在131例抗-HBe阳性血清中的81例（62%）中检测到（斑点印迹法为5%），在61例HBe标志物阴性者中的29例（48%）中检测到（斑点印迹法为3%），在21例δ阳性血清中的6例（29%）中检测到。在HBs-Ag阴性患者中，27例抗-HBc阳性血清中的6例（22%）检测到HBV DNA，但在抗-HBc和抗-HBs均阳性的血清（n = 50）中未检测到，在患有肝病的HBV标志物阴性患者（n = 30）和健康对照者（n = 50）中也未检测到。如果要检查传染性或存在双重感染的情况，推荐使用更敏感的聚合酶链反应来检测HBV DNA。HBe-Ag阳性患者必须被视为具有传染性：一般可省略HBV DNA检测。
