Shibasaki T, Yamada K, Yamauchi N, Imaki T, Hotta M, Demura H
Department of Medicine, Tokyo Women's Medical College, Japan.
Neurosci Lett. 1994 Oct 24;180(2):167-70. doi: 10.1016/0304-3940(94)90513-4.
It has been shown that 1 h restraint shortens pentobarbital (PbNa)-induced sleeping time and that brain corticotropin-releasing hormone (CRH) is involved in the mechanism by which restraint shortens. PbNa-induced sleeping time. The present study was designed to further examine the mechanism of the antagonistic effect of 1 h restraint on PbNa in rats. Intracerebroventricular (i.c.v.) administration of propranolol and metoprolol, but not butoxamine reversed the shortening of PbNa-induced sleeping time by 1 h restraint. The i.c.v. administration of phentolamine blocked the shortening of PbNa-induced sleeping time by restraint, while the same dose of phentolamine prolonged the sleeping time in unrestrained rats. Atropine did not affect the PbNa-induced sleeping time in restrained rats. These results suggest that in addition to CRH, the brain beta 1-adrenergic system is involved in the restraint stress-induced increase in arousal.
研究表明，1小时的束缚会缩短戊巴比妥（PbNa）诱导的睡眠时间，且脑促肾上腺皮质激素释放激素（CRH）参与了束缚缩短睡眠时间的机制。本研究旨在进一步探讨1小时束缚对大鼠PbNa拮抗作用的机制。脑室内（i.c.v.）注射普萘洛尔和美托洛尔可逆转1小时束缚导致的PbNa诱导睡眠时间缩短，但布托沙明则无此作用。脑室内注射酚妥拉明可阻断束缚导致的PbNa诱导睡眠时间缩短，而相同剂量的酚妥拉明却延长了未受束缚大鼠的睡眠时间。阿托品对受束缚大鼠的PbNa诱导睡眠时间无影响。这些结果表明，除CRH外，脑β1-肾上腺素能系统也参与了束缚应激诱导的觉醒增加。
