Adén U, Bona E, Hagberg H, Fredholm B B
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Neurosci Lett. 1994 Oct 24;180(2):91-5. doi: 10.1016/0304-3940(94)90495-2.
We used quantitative in situ hybridization to study changes in the expression of c-fos following hypoxic-ischemia (H-I) in the neonatal rat brain. 7-day-old rat pups were subjected to a unilateral ligation of the common carotid artery followed by a 2 h 15 min hypoxic period (7.7% O2 in N2). This resulted in the expected ipsilateral infarction of cortex, lateral hippocampus, lateral-superior aspects of the striatum and the white matter of the corpus callosum. Brain damage was not seen in the contralateral hemisphere subjected only to hypoxia. c-fos mRNA levels increased in the contralateral hemisphere immediately after the hypoxia and had returned towards normal levels 2 h thereafter. In the ipsilateral hemisphere, the expression of c-fos was delayed but very marked at 2 h. Animals subjected only to hypoxia showed little or no increase in c-fos mRNA. Thus the earliest recorded increase in c-fos after hypoxic ischemia, which occurred on the non-ischemic, contralateral side, may represent a generalized response to a more localized insult.
我们采用定量原位杂交技术，研究新生大鼠脑缺氧缺血（H-I）后c-fos表达的变化。对7日龄的幼鼠进行单侧颈总动脉结扎，随后进行2小时15分钟的缺氧处理（氮气中含7.7%氧气）。这导致了预期的同侧皮质、外侧海马、纹状体外侧上部以及胼胝体白质梗死。仅经历缺氧的对侧半球未见脑损伤。缺氧后立即，对侧半球的c-fos mRNA水平升高，并在2小时后恢复至正常水平。在同侧半球，c-fos的表达延迟，但在2小时时非常明显。仅经历缺氧的动物c-fos mRNA几乎没有增加或没有增加。因此，缺氧缺血后最早记录到的c-fos增加发生在非缺血的对侧，这可能代表了对更局部损伤的全身性反应。
