Dores R M, Gieseker K, Steveson T C
University of Denver, Department of Biological Sciences, CO 80208.
Peptides. 1994;15(8):1497-504. doi: 10.1016/0196-9781(94)90129-5.
Fractionation of an acid extract of 15 B. marinus intermediate pituitaries by a combination of gel filtration chromatography and cation exchange chromatography revealed one major and five minor forms of beta-endorphin in this tissue. Based on reversed-phase HPLC and immunological properties, as well as amino acid composition and primary sequence analysis, it was deduced that the sequence of the major form of B. marinus beta-endorphin is N-acetyl-YGGFMTPE. Overall, the steady-state analyses of the minor forms of beta-endorphin indicated that the posttranslational processing of beta-endorphin in the toad intermediate pituitary includes endoproteolytic cleavage at both paired basic and monobasic cleavage sites.
通过凝胶过滤色谱法和阳离子交换色谱法相结合，对15个海蟾蜍中间垂体的酸性提取物进行分级分离，结果显示该组织中存在一种主要形式和五种次要形式的β-内啡肽。基于反相高效液相色谱法、免疫学特性以及氨基酸组成和一级序列分析，推断出海蟾蜍β-内啡肽主要形式的序列为N-乙酰基-YGGFMTPE。总体而言，对β-内啡肽次要形式的稳态分析表明，蟾蜍中间垂体中β-内啡肽的翻译后加工包括在成对碱性和单碱性切割位点的内蛋白水解切割。
