Knöfler R, Urano T, Malyszko J, Takada Y, Takada A
Department of Physiology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Thromb Res. 1995 Jan 1;77(1):69-78. doi: 10.1016/0049-3848(95)90866-e.
The effect of ET-1 on ADP- and collagen-induced platelet aggregation in whole blood and platelet rich plasma (PRP) was studied in 39 healthy volunteers. Although ET-1 itself did not cause platelet aggregation, a marked enhancement of ADP-induced aggregation after the preincubation with ET-1 for 5 min was observed in whole blood, but not in PRP. This ET-1 concentration and preincubation time-dependent phenomenon could be demonstrated only at threshold concentrations (5 and 7.5 microM) of ADP and is probably due to an interaction of ET-1 with cells which are involved in the whole blood aggregation, such as polymorphonuclear neutrophils. In whole blood and PRP an inhibition of collagen-induced aggregation after the preincubation with ET-1 was detected. In contrast to ADP, a direct influence of ET-1 on platelet activation after the addition of collagen is therefore more likely. These results suggest that human platelets may possess ET-1 receptor(s) and that ET-1 may also interact with other blood cells.
在39名健康志愿者中研究了内皮素-1（ET-1）对全血和富血小板血浆（PRP）中ADP和胶原诱导的血小板聚集的影响。尽管ET-1本身不会引起血小板聚集，但在全血中，ET-1预孵育5分钟后，可观察到ADP诱导的聚集显著增强，而在PRP中则未观察到。这种ET-1浓度和预孵育时间依赖性现象仅在ADP的阈值浓度（5和7.5 microM）时出现，可能是由于ET-1与参与全血聚集的细胞（如多形核中性粒细胞）相互作用所致。在全血和PRP中，ET-1预孵育后可检测到胶原诱导的聚集受到抑制。与ADP不同，因此ET-1在添加胶原后对血小板活化的直接影响更有可能。这些结果表明，人血小板可能具有ET-1受体，并且ET-1也可能与其他血细胞相互作用。
