Stupina A S, Terman A K, Kvitnitskaia-Ryzhova T Iu, Mezhiborskaia N A, Zherebitskiĭ V A
Tsitol Genet. 1994 Nov-Dec;28(6):15-20.
To elucidate the role of autophagy in age-related lipofuscinogenesis, the accumulation of autophagic vacuoles (AV) caused by the blockade of their fusion with lysosomes under the effect of vinblastine was studied in cells of various tissues (hepatocytes, pinealocytes, epitheliocytes of choroid plexus, cardiomyocytes, neurons) in mice and rats of different ages. The degree of AV accumulation in cells of old animals did not exceed that in adult ones. The results suggest that the accumulation of lipofuscin (LF) in aging cells is mainly due to the disturbance of lysosomal degradation or removal of LF out of cells rather than to the increase in autophagy.
为了阐明自噬在与年龄相关的脂褐素生成中的作用，研究了在长春碱作用下，自噬泡（AV）与溶酶体融合受阻所导致的自噬泡在不同年龄小鼠和大鼠的各种组织细胞（肝细胞、松果体细胞、脉络丛上皮细胞、心肌细胞、神经元）中的积累情况。老年动物细胞中自噬泡的积累程度不超过成年动物。结果表明，衰老细胞中脂褐素（LF）的积累主要是由于溶酶体降解功能的紊乱或脂褐素从细胞中清除受阻，而非自噬增加所致。
