[利用随机引物PCR技术展示的谷物遗传多态性]
[The genetic polymorphism of cereals demonstrated by PCR with random primers].
作者信息
Sivolap Iu M, Kalendar' R N, Chebotar' S V
出版信息
Tsitol Genet. 1994 Nov-Dec;28(6):54-61.
PMID:7701604
Abstract
Polymerase chain reaction of DNA amplification with the use of random primers is a new approach in investigations of genome specificity. Nucleotide sequences that showed polymorphism in RFLP analysis were used as primers. Optimal temperature conditions and Mg2+ concentrations were determined for studying inter- and intraspecific DNA polymorphism in the most important cereals.
摘要
使用随机引物进行DNA扩增的聚合酶链反应是基因组特异性研究中的一种新方法。在限制性片段长度多态性（RFLP）分析中显示出多态性的核苷酸序列被用作引物。确定了最佳温度条件和镁离子浓度，用于研究最重要谷物的种间和种内DNA多态性。
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