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利用随机扩增多态性DNA聚合酶链反应对马铃薯品种和栽培种进行指纹图谱分析

[Fingerprinting of potato species and cultivar using RAPD-PCR].

作者信息

Oganisian A S, Kochieva E Z, Ryskov A P

出版信息

Genetika. 1996 Mar;32(3):448-51.

PMID:8723639
Abstract

Polymerase chain reaction with random primers (RAPD-PCR) is used to fingerprint DNA polymorphism of six species and eight cultivars of potato. Three primers were developed experimentally for detection by DNA fingerprinting of cultivars and species. High significance and reproducibility of amplified DNA spectra for each species and cultivar was demonstrated. In these spectra, RAPD-PCR fragments, were revealed which may serve as cultivar-and species-specific markers for identification and differentiation of Solanum cultivars and species.

摘要

随机引物聚合酶链反应(RAPD-PCR)用于对六个马铃薯品种和八个马铃薯栽培种的DNA多态性进行指纹分析。通过实验开发了三种引物,用于通过DNA指纹分析来检测栽培种和品种。每个品种和物种的扩增DNA谱具有高度的显著性和可重复性。在这些谱图中,发现了RAPD-PCR片段,这些片段可作为栽培种和物种特异性标记,用于鉴定和区分茄属栽培种和物种。

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