从黏粒中分离出的猪微卫星显示出多态性并定位在染色体上。
Pig microsatellites isolated from cosmids revealing polymorphism and localized on chromosomes.
作者信息
Robic A, Parrou J L, Yerle M, Goureau A, Dalens M, Milan D, Gellin J
机构信息
INRA, Laboratoire de Génétique Cellulaire, Castanet, Tolosan, France.
出版信息
Anim Genet. 1995 Feb;26(1):1-6. doi: 10.1111/j.1365-2052.1995.tb02611.x.
One of the most widely studied simple sequences in the mammalian genome is the (TG)n dinucleotide sequence. Because these microsatellites are highly polymorphic, we chose to study microsatellites from cosmids to provide genetic markers for the porcine genome. After screening a porcine cosmid library with a (CA)10 probe, 20 cosmids containing microsatellites were subcloned and 17 microsatellites identified by sequencing. Oligonucleotide primers flanking the repeat were designed for seven (TG)n microsatellites with n > 14. These seven microsatellites revealed polymorphism and were regionally assigned to chromosomes by fluorescent in situ hybridization of initial cosmids. These seven loci will be useful for both the construction of the genetic map and as landmark loci on the physical map of the porcine genome.
哺乳动物基因组中研究最为广泛的简单序列之一是（TG）n二核苷酸序列。由于这些微卫星具有高度多态性，我们选择研究来自黏粒的微卫星，以便为猪基因组提供遗传标记。用（CA）10探针筛选猪黏粒文库后，对20个含有微卫星的黏粒进行亚克隆，并通过测序鉴定出17个微卫星。针对7个n>14的（TG）n微卫星，设计了位于重复序列两侧的寡核苷酸引物。这7个微卫星显示出多态性，并通过最初黏粒的荧光原位杂交将其区域定位到染色体上。这7个基因座对于构建遗传图谱以及作为猪基因组物理图谱上的标志性基因座都将是有用的。
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