Robic A, Riquet J, Yerle M, Milan D, Lahbib-Mansais Y, Dubut-Fontana C, Gellin J
INRA Laboratoire de Génétique Cellulaire, BP27, 31326 Castanet Tolosan Cedex, France.
Mamm Genome. 1996 Jun;7(6):438-45. doi: 10.1007/s003359900129.
Recently two main genetic maps [Rohrer et al. Genetics 136, 231 (1994); Archibald et al. Mamm. Genome 6, 157 (1995)] and a cytogenetic map [Yerle et al. Mamm. Genome 6, 175 (1995)] for the porcine genome were reported. As only a very few micro-satellites are located on the cytogenetic map, it appears to be important to increase the relationships between the genetic and cytogenetic maps. This document describes the regional mapping of 100 genetic markers with a somatic cell hybrid panel. Among the markers, 91 correspond to new localizations. Our study enabled the localization of 14 new markers found on both maps, of 54 found on the USDA map, and of 23 found on the PiGMaP map. Now 21% and 43% of the markers on the USDA and PiGMaP linkage maps respectively are physically mapped. This new cytogenetic information was then integrated within the framework of each genetic map. The cytogenetic orientation of the USDA linkage maps for Chromosomes (Chrs) 3, 8, 9, and 16 and of PiGMaP for Chr 8 was determined. USDA and PiGMaP linkage maps are now oriented for all chromosomes, except for Chrs 17 and 18. Moreover, the linkage group "R" from the USDA linkage map was assigned to Chr 6.
最近报道了两份主要的猪基因组遗传图谱[罗勒等人，《遗传学》136卷，231页（1994年）；阿奇博尔德等人，《哺乳动物基因组》6卷，157页（1995年）]和一份细胞遗传学图谱[耶勒等人，《哺乳动物基因组》6卷，175页（1995年）]。由于细胞遗传学图谱上仅定位了极少数微卫星，因此增强遗传图谱与细胞遗传学图谱之间的关联显得尤为重要。本文描述了利用体细胞杂种板对100个遗传标记进行区域定位的情况。在这些标记中，91个对应新的定位。我们的研究实现了对在两份图谱上均发现的14个新标记、在美国农业部（USDA）图谱上发现的54个标记以及在猪基因图谱（PiGMaP）上发现的23个标记的定位。现在，USDA和PiGMaP连锁图谱上分别有21%和43%的标记已进行物理定位。然后，这些新的细胞遗传学信息被整合到每个遗传图谱的框架内。确定了USDA连锁图谱中第3、8、9和16号染色体以及PiGMaP中第8号染色体的细胞遗传学方向。除了第17和18号染色体外，USDA和PiGMaP连锁图谱现在已确定了所有染色体的方向。此外，USDA连锁图谱中的连锁群“R”被定位到第6号染色体。
