从黏粒中直接鉴定牛微卫星：多态性和同线基因定位

Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping.

作者信息

Ferretti L, Leone P, Pilla F, Zhang Y, Nocart M, Guérin G

机构信息

Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Milano, Italy.

出版信息

Anim Genet. 1994 Aug;25(4):209-14. doi: 10.1111/j.1365-2052.1994.tb00195.x.

DOI:10.1111/j.1365-2052.1994.tb00195.x

PMID:7985836

Abstract

A (TG)8 oligonucleotide probe was used to screen 186 cosmids from a commercial bovine cosmid library. Of the 56 positive discovered, 7 were sequenced in the region of the microsatellite and analysed for polymorphism. These microsatellites, IDVGA-2, -3, -7, -8, -9, -10, -11 showed the following number of alleles and polymorphism information content (PIC) values (7/0.616, 8/0.693, 6/0.641, 5/0.643, 2/0.239, 10/0.844, 6/0.720). The microsatellites were also assigned to synteny groups as follows: IDVGA-2/U17, IDVGA-3/U16, IDVGA-7/U7, IDVGA-8/U29, IDVGA-9/U3, IDVGA-10/U19, IDVGA-11/A (probably U18).

摘要

使用一个（TG）8寡核苷酸探针从一个商业牛黏粒文库中筛选186个黏粒。在发现的56个阳性克隆中，对其中7个在微卫星区域进行测序并分析其多态性。这些微卫星，即IDVGA - 2、- 3、- 7、- 8、- 9、- 10、- 11，显示出以下等位基因数量和多态性信息含量（PIC）值（7/0.616、8/0.693、6/0.641、5/0.643、2/0.239、10/0.844、6/0.720）。这些微卫星还被分配到以下同线群：IDVGA - 2/U17、IDVGA - 3/U16、IDVGA - 7/U7、IDVGA - 8/U29、IDVGA - 9/U3、IDVGA - 10/U19、IDVGA - 11/A（可能是U18）。