Crerar S K, Cross G M
Department of Animal Health, University of Sydney, Camden, New South Wales.
Aust Vet J. 1994 Dec;71(12):414-7. doi: 10.1111/j.1751-0813.1994.tb00958.x.
Big liver and spleen disease (BLS) was reproduced experimentally by intravenous (IV) and oral (PO) administration of BLS inocula to susceptible broiler breeder hens 34 to 36 weeks of age. Serological and pathological signs of BLS similar to those seen in the natural disease occurred in inoculated and in-contact birds. Splenomegaly was the earliest and often the only necropsy finding, with hepatomegaly and kidney enlargement occurring in some birds later in the course of the disease. After IV administration, serum antigen was detected between 2 and 4 weeks, and antibody between 3 and 5 weeks. After PO administration, antigen was detected between 2 and 4 weeks, and antibody between 3 and 6 weeks. Antibody persisted in all birds to the end of the experiment (6 weeks), and horizontal transmission probably occurred since in-contact birds developed BLS. Liver probably contained the highest concentration of BLS agent because it had the highest infectivity.
通过给34至36周龄的易感肉种鸡静脉注射（IV）和口服（PO）接种物，成功复制了大肝脾病（BLS）。接种和接触的鸡出现了与自然疾病相似的BLS血清学和病理学症状。脾肿大是最早出现且通常是唯一的尸检发现，在病程后期，部分鸡出现肝肿大和肾肿大。静脉注射后，2至4周可检测到血清抗原，3至5周可检测到抗体。口服给药后，2至4周可检测到抗原，3至6周可检测到抗体。所有鸡的抗体持续到实验结束（6周），由于接触的鸡出现了BLS，可能发生了水平传播。肝脏可能含有最高浓度的BLS病原体，因为它具有最高的传染性。
