Kanai Y, Ochiai A, Shibata T, Oyama T, Ushijima S, Akimoto S, Hirohashi S
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Mar 28;208(3):1067-72. doi: 10.1006/bbrc.1995.1443.
Association of the c-erbB-2 oncogene product with the cadherin-catenin complex has been demonstrated in human cancer cell lines. Although beta-catenin and plakoglobin have been proven to be crucial for the association, no previous study has shown whether the interactions are direct or indirect. In the present study, the c-erbB-2 gene product was shown by far-Western blotting analysis to associate directly with both beta-catenin and plakoglobin through its cytoplasmic domain core region, which showed extensive homology with epidermal growth factor receptor. These data suggest that c-erbB-2-induced signaling is also directly liked to the cadherin-mediated cell adhesion and "invasion-suppressor" system through beta-catenin and plakoglobin in cancers.
在人类癌细胞系中已证实c-erbB-2癌基因产物与钙黏蛋白-连环蛋白复合物有关联。尽管已证明β-连环蛋白和桥粒斑珠蛋白对于这种关联至关重要，但此前尚无研究表明这些相互作用是直接的还是间接的。在本研究中，通过远缘Western印迹分析表明，c-erbB-2基因产物通过其细胞质结构域核心区域与β-连环蛋白和桥粒斑珠蛋白直接关联，该区域与表皮生长因子受体具有广泛的同源性。这些数据表明，在癌症中，c-erbB-2诱导的信号传导也通过β-连环蛋白和桥粒斑珠蛋白与钙黏蛋白介导的细胞黏附及“侵袭抑制”系统直接相关。
