Bianchi R, Garbuglia M, Verzini M, Giambanco I, Spreca A, Donato R
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Biochem Biophys Res Commun. 1995 Mar 28;208(3):910-8. doi: 10.1006/bbrc.1995.1421.
S-100 protein and annexin II2-p11(2) were reported to inhibit and to stimulate the assembly of glial fibrillary acidic protein (GFAP), respectively, in a Ca(2+)-dependent manner. Here we show by a number of experimental approaches that S-100 protein contrasts all the effects of annexin II2-p11(2) on GFAP assembly and, conversely, that annexin II2-p11(2) contrasts the inhibitory effects of S-100 protein on GFAP assembly, in a dose-dependent manner in both cases. Altogether, these data suggest that two specific Ca2+ effectors, i.e., annexin II2-p11(2) and S-100 protein, might regulate the state of assembly of glial filaments in a concerted manner.
据报道，S-100蛋白和膜联蛋白II2-p11(2)分别以Ca(2+)依赖的方式抑制和刺激胶质纤维酸性蛋白（GFAP）的组装。在这里，我们通过多种实验方法表明，S-100蛋白与膜联蛋白II2-p11(2)对GFAP组装的所有影响相反，反之亦然，膜联蛋白II2-p11(2)与S-100蛋白对GFAP组装的抑制作用相反，在两种情况下均呈剂量依赖性。总之，这些数据表明，两种特定的Ca2+效应物，即膜联蛋白II2-p11(2)和S-100蛋白，可能以协同方式调节胶质丝的组装状态。
