胶质纤维酸性蛋白（GFAP）在结节性硬化症患者培养细胞中间丝中的分布。

Distribution of glial fibrillary acidic protein (GFAP) in the intermediate filaments of the cultured cells from a patient with tuberous sclerosis.

作者信息

Onodera K, Takahashi T, Watanabe R, Ishibashi Y, Sasaki M, Kimura G

机构信息

Department of Agricultural Chemistry, University of Tokyo, Japan.

出版信息

J Dermatol. 1990 Jul;17(7):395-402. doi: 10.1111/j.1346-8138.1990.tb01665.x.

DOI:10.1111/j.1346-8138.1990.tb01665.x

PMID:2229641

Abstract

We established a cell line (TS) from adenoma sebaceum of a patient with tuberous sclerosis. Through our previous studies, the abnormal cell division and dysfunction of TS cells were indicated. Glial fibrillary acidic protein (GFAP) and 55 kd protein had been to be major cytoskeletal proteins in these cells. This time we have examined the structure and distribution of cytoskeletons in TS cells with immunoelectron microscopy. TS cells were found to coexpress GFAP and vimentin-like substance; both structures seem to be closely related. The coexistence of a few kinds of proteins integrated in the cytoskeletons might lead to the abnormal behavior of the nucleus during the process of cell division.

摘要

我们从一名结节性硬化症患者的皮脂腺腺瘤中建立了一个细胞系（TS）。通过我们之前的研究，已表明TS细胞存在异常细胞分裂和功能障碍。胶质纤维酸性蛋白（GFAP）和55kd蛋白曾被认为是这些细胞中的主要细胞骨架蛋白。此次我们用免疫电子显微镜检查了TS细胞中细胞骨架的结构和分布。发现TS细胞共表达GFAP和波形蛋白样物质；两者结构似乎密切相关。细胞骨架中几种蛋白质的共存可能导致细胞分裂过程中细胞核的异常行为。