Lennard A, Gaston K, Fried M
Eukaryotic Gene Organisation and Expression Laboratory, Imperial Cancer Research Fund, London, UK.
DNA Cell Biol. 1994 Nov;13(11):1117-26. doi: 10.1089/dna.1994.13.1117.
The organization of the Surfeit locus and the juxtaposition of at least five of the Surfeit genes (Surf-1 to -5) are conserved between mouse and human (Williams et al., 1988; Yon et al., 1993). In the mouse, the heterogeneous transcription start sites of the divergent Surf-1 and Surf-2 genes are separated by a maximum of only 73 bp (Williams and Fried, 1986). This region contains a bidirectional promoter composed of three major factor binding sites required for the efficient expression of both the Surf-1 and Surf-2 genes (Lennard and Fried, 1991). Here we report the isolation and characterization of the human Surf-1 and Surf-2 genes and their intergenic region. Although the major Surf-1 and Surf-2 transcription start sites are separated by 97 bp in the human and there are multiple differences in the mouse and human sequence between and around the transcriptional start sites, there is high conservation of the sequence specifying the three major factor binding sites of the bidirectional promoter. The three factor binding sites (HSu1, 2, and 3) present within the human promoter bind nuclear factors, of which the binding of HSu1 and HSu2 are competed by oligonucleotides carrying the corresponding mouse factor binding sites. The HSu3 site binds factors that are similar but apparently not direct homologs of those that bind to the equivalent mouse sequences. Human Surf-1 and Surf-2 cDNAs have been cloned and sequenced. The putative human Surf-1 and Surf-2 proteins are 77% and 69% identical to the corresponding mouse proteins.
在小鼠和人类之间，Surfeit基因座的组织以及至少五个Surfeit基因（Surf-1至Surf-5）的并置是保守的（Williams等人，1988年；Yon等人，1993年）。在小鼠中，反向排列的Surf-1和Surf-2基因的异质转录起始位点之间最多仅相隔73 bp（Williams和Fried，1986年）。该区域包含一个双向启动子，由Surf-1和Surf-2基因高效表达所需的三个主要因子结合位点组成（Lennard和Fried，1991年）。在此，我们报告了人类Surf-1和Surf-2基因及其基因间区域的分离和特征。尽管人类中主要的Surf-1和Surf-2转录起始位点相隔97 bp，并且在转录起始位点之间及其周围的小鼠和人类序列存在多个差异，但指定双向启动子三个主要因子结合位点的序列具有高度保守性。人类启动子中存在的三个因子结合位点（HSu1、2和3）结合核因子，其中HSu1和HSu2的结合被携带相应小鼠因子结合位点的寡核苷酸竞争。HSu3位点结合的因子与结合等效小鼠序列的因子相似，但显然不是直接同源物。人类Surf-1和Surf-2 cDNA已被克隆和测序。推测的人类Surf-1和Surf-2蛋白与相应的小鼠蛋白分别具有77%和69%的同一性。
