Gaston K, Fried M
Department of Biochemistry, University of Bristol, School of Medical Sciences, UK.
FEBS Lett. 1994 Jun 27;347(2-3):289-94. doi: 10.1016/0014-5793(94)00567-2.
The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains three important factor binding sites: Su1, Su2, and Su3. These sites are conserved between the mouse and human Surf-1/Surf-2 promoters, bind nuclear factors in vitro, and are required for accurate and efficient expression of Surf-1 and Surf-2 in vivo. Using gel retardation assays, methylation interference experiments, and specific antibodies we demonstrate that the Su1 binding factor is the initiator protein YY1. Over-expression of YY1 results in a major stimulation of transcription in the Surf-1 direction and a minor stimulation of transcription in the Surf-2 direction.
过量基因座是一个不同寻常的基因簇，至少包含6个管家基因，其基因组织在鸟类和哺乳动物之间是保守的。我们之前已经表明，反向转录的Surf-1和Surf-2基因由一个无TATA盒的双向启动子分隔开。在小鼠中，Surf-1/Surf-2启动子包含三个重要的因子结合位点：Su1、Su2和Su3。这些位点在小鼠和人类的Surf-1/Surf-2启动子之间是保守的，能在体外结合核因子，并且是Surf-1和Surf-2在体内准确高效表达所必需的。通过凝胶阻滞分析、甲基化干扰实验和特异性抗体，我们证明Su1结合因子是起始蛋白YY1。YY1的过表达导致Surf-1方向的转录受到主要刺激，而Surf-2方向的转录受到轻微刺激。
