在制备型超速离心机中使用不连续梯度快速分离质粒DNA。
Fast separations of plasmid DNA using discontinuous gradients in the preparative ultracentrifuge.
作者信息
Dorin M, Bornecque C A
机构信息
Research and Applications Department, Beckman Instruments, Palo Alto, CA 94304.
出版信息
Biotechniques. 1995 Jan;18(1):90, 92.
PMID:7702860
Abstract
Plasmid DNA can be purified in 20 min using a three-step CsCl gradient in a vertical tube rotor. Ultracentrifugation with discontinuous gradients yields DNA with purity comparable to that obtained with longer isopycnic separations and with columns.
摘要
使用垂直管转子中的三步氯化铯梯度，可在20分钟内纯化质粒DNA。采用不连续梯度进行超速离心所获得的DNA纯度，与通过较长时间的等密度分离和柱层析所获得的DNA纯度相当。
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