Fonarev A B, Shugaliĭ A V
Ukr Biokhim Zh. 1977 Mar-Apr;49(2):96-102.
Behaviour of calf thymus heterogenous DNA was studied in the angular rotor Ti50 of the preparation ultracentrifuge. A drop in DNA polymericity from 8-10(6) to 4-10(5) daltons and decrease in the amount of the applied material result in detecting the known satellite component with delta1 = 1.714 g/cm3, the value of the floating density of the main peak takes on the "true" value delta = 1.699 g/cm3. The method of digging up affects the symmetry of polymeric sample profiles. The satellite component is not found when digging up from the tube bottom. The values of GG content of the light and "heavy" fractions calculated from their floating densities during repeated centrifugation in the angular rotor and from fusing temperatures are in good agrrement.
在制备超速离心机的角转子Ti50中研究了小牛胸腺异源DNA的行为。DNA聚合度从8 - 10(6)道尔顿降至4 - 10(5)道尔顿，以及所加材料量的减少，导致检测到已知的卫星组分，其δ1 = 1.714 g/cm3，主峰的漂浮密度值呈现“真实”值δ = 1.699 g/cm3。挖掘方法会影响聚合物样品谱的对称性。从管底部挖掘时未发现卫星组分。在角转子中重复离心期间，根据轻、“重”组分的漂浮密度以及熔合温度计算出的GG含量值吻合良好。
