Vlaemynck G, Herman L, Coudijzer K
Dairy Research Institute, Melle, Belgium.
Int J Food Microbiol. 1994 Dec;24(1-2):211-25. doi: 10.1016/0168-1605(94)90120-1.
A total of 4000 bacterial strains were isolated from milk, cheese and from different samples taken on the farm (silage, faeces) and screened for their antimicrobial activity against Listeria monocytogenes. Only eight of the almost 4000 strains exhibited inhibitory activity in the cell-free supernatant fluid. Two different Enterococcus strains (RZS C5 and RZS C13) were chosen for further study. Plasmid profile analysis of both revealed that RZS C5 contained a 4 kb plasmid while RZS C13 contained a 23 kb plasmid. The inhibitory compounds were proteinaceous and active against all Listeria spp. tested while most of the lactic acid bacteria were insensitive. Weak inhibition was also detected against Clostridium perfringens and some Bacillus spp. Cross-inhibition tests showed different profiles. The bacteriocins were heat-resistant and were very stable during storage, especially at low pH values. Their molecular weight was estimated at +/- 3.0 kDA. Addition of 200 AU/ml of bacteriocin resulted in an initial rapid decrease of the number of viable L. monocytogenes cells followed by slower a logarithmic decrease.
从牛奶、奶酪以及农场采集的不同样本（青贮饲料、粪便）中总共分离出4000株细菌菌株，并对它们针对单核细胞增生李斯特菌的抗菌活性进行筛选。在近4000株菌株中，只有8株在无细胞上清液中表现出抑制活性。选择了两株不同的肠球菌菌株（RZS C5和RZS C13）进行进一步研究。对两者的质粒图谱分析表明，RZS C5含有一个4 kb的质粒，而RZS C13含有一个23 kb的质粒。抑制性化合物是蛋白质类的，对所有测试的李斯特菌属均有活性，而大多数乳酸菌不敏感。对产气荚膜梭菌和一些芽孢杆菌属也检测到微弱抑制作用。交叉抑制试验显示出不同的图谱。这些细菌素耐热，在储存期间非常稳定，尤其是在低pH值下。它们的分子量估计为±3.0 kDA。添加200 AU/ml的细菌素导致单核细胞增生李斯特菌活菌数最初迅速下降，随后呈较慢的对数下降。
