Nakamura T, Sato T O, Miiamoto S
Biofizika. 1995 Jan-Feb;40(1):185-91.
We have examined the membrane conductance in the frog olfactory receptor cilia by the "whole cilia patch" method according to Kleen and Gesteland [1]. We always observed the cyclic AMP-gated conductance as reported previously on the excited inside-out membrane patch from the cilium. However, we could not detect a significant indication of the IP3-gated conductance in the ciliary membrane. On the contrary, Ca(2+)-gated chloride conductance was detected in most cilia. The mean value of this conductance was more than 10% of the cAMP-gated conductance measured in divalent cation free solution. This result may indicate the certain role of the chloride channel in the generation of the olfactory receptor potential.
我们根据克林和格斯特兰[1]的方法，采用“全纤毛膜片”技术检测了蛙嗅觉受体纤毛的膜电导。正如之前在纤毛外翻膜片上所报道的那样，我们始终观察到环磷酸腺苷（cAMP）门控电导。然而，我们在纤毛膜中未检测到明显的肌醇三磷酸（IP3）门控电导迹象。相反，在大多数纤毛中检测到了钙离子（Ca²⁺）门控氯离子电导。该电导的平均值超过了在无二价阳离子溶液中测得的cAMP门控电导的10%。这一结果可能表明氯离子通道在嗅觉受体电位产生过程中具有一定作用。
