Seroogy K B, Numan S, Gall C M, Lee D C, Kornblum H I
Department of Anatomy and Neurobiology, University of Kentucky, Lexington 40536-0084, USA.
Neuroreport. 1994 Dec 30;6(1):105-8. doi: 10.1097/00001756-199412300-00028.
In situ hybridization with a cRNA probe was used to determine the cellular expression of mRNA for the epidermal growth factor receptor (EGF-R) within the rat dopaminergic nigrostriatal system. Hybridization for EGF-R mRNA was distributed throughout the ventral mesencephalon within cells of the substantia nigra pars compacta and ventral tegmental area (VTA). Unilateral lesion of the nigrostriatal pathway with the neurotoxin 6-hydroxydopamine resulted in substantial elimination of EGF-R cRNA hybridization in the ipsilateral substantia nigra and VTA. EGF-R mRNA was also demonstrated within a population of striatal somata, with highest levels of expression detected neonatally. These results indicate that a subpopulation of ventral midbrain dopaminergic neurons, as well as striatal perikarya, express the EGF-R and, thus, may be directly responsive to endogenous EGF, transforming growth factor-alpha, or another EGF-R ligand in vivo.
采用与cRNA探针的原位杂交技术，以确定大鼠多巴胺能黑质纹状体系统内表皮生长因子受体（EGF-R）的mRNA细胞表达情况。EGF-R mRNA的杂交分布于整个腹侧中脑黑质致密部和腹侧被盖区（VTA）的细胞内。用神经毒素6-羟基多巴胺对黑质纹状体通路进行单侧损伤，导致同侧黑质和VTA中EGF-R cRNA杂交显著消除。在一群纹状体胞体中也证实了EGF-R mRNA的存在，在新生期检测到最高表达水平。这些结果表明，腹侧中脑多巴胺能神经元亚群以及纹状体周核表达EGF-R，因此在体内可能直接对内源性EGF、转化生长因子-α或其他EGF-R配体产生反应。
