Catt J W
Human Reproduction Unit, Royal North Shore Hospital, St. Leonards, New South Wales, Australia.
Cell Biol Int. 1994 Dec;18(12):1155-62. doi: 10.1006/cbir.1994.1042.
The three methods of culturing bovine zygotes; surrogate oviducts, co-culture and defined media are compared and contrasted. Surrogate oviducts provide the best environment and so provide a benchmark to compare in vitro culture systems. At present, co-culture systems provide a convenient method of reliably producing embryos suitable for transfer. This is important for commercial embryo production. The use of defined media is improving and can be as efficacious as co-culture. The advantage of defined culture systems is the ability to find the requirements of an early embryo and to tailor media to meet them. It is suggested that maturation, not zygote culture, may be the constraint in embryonic development.
对培养牛受精卵的三种方法；代用输卵管、共培养和限定培养基进行了比较和对比。代用输卵管提供了最佳环境，因此为比较体外培养系统提供了一个基准。目前，共培养系统提供了一种可靠地生产适合移植胚胎的便捷方法。这对商业胚胎生产很重要。限定培养基的使用正在改进，其效果可与共培养相当。限定培养系统的优点是能够找出早期胚胎的需求，并定制培养基以满足这些需求。有人认为，胚胎发育的限制因素可能是成熟过程，而非受精卵培养。
