Terui J, Tamoto K, Sudo J
Department of Clinical Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Biol Pharm Bull. 1994 Nov;17(11):1516-8. doi: 10.1248/bpb.17.1516.
To compare proteinuric potentials among angiotensin II (ANG II) and its fragments, [des-Asp1]-angiotensin II (ANG III) and [des-Asp1, des-Arg2]-angiotensin II (ANG IV), the peptide was intravenously infused for 30 min at doses of 0.015, 0.05, 0.15, 0.45 and 1.45 nmol/kg body weight/min. The infusion of ANG II and ANG III increased the fractional clearance of albumin in a dose-dependent manner: most extensively for ANG II, and moderately for ANG III. In contrast, the infusion of ANG IV hardly showed any proteinuric action, even at the maximal dose of 1.45 nmol/kg body weight/min. These results denoted that the cleaving of the N-terminal aspartic acid1 from ANG II weakened the proteinuric action in the glomeruli, and the further cleaving of the N-terminal arginine from ANG III led to a complete loss of proteinuric action in the glomeruli.
为比较血管紧张素II（ANG II）及其片段[去天冬氨酸1] - 血管紧张素II（ANG III）和[去天冬氨酸1，去精氨酸2] - 血管紧张素II（ANG IV）的蛋白尿生成潜力，以0.015、0.05、0.15、0.45和1.45 nmol/（kg体重·分钟）的剂量静脉输注该肽30分钟。ANG II和ANG III的输注以剂量依赖性方式增加白蛋白的分数清除率：ANG II作用最显著，ANG III作用中等。相比之下，即使在最大剂量1.45 nmol/（kg体重·分钟）时，ANG IV的输注也几乎未显示出任何蛋白尿作用。这些结果表明，从ANG II上切割掉N端天冬氨酸1会减弱肾小球中的蛋白尿作用，而从ANG III上进一步切割掉N端精氨酸会导致肾小球中蛋白尿作用完全丧失。
