Jacob H J, Brown D M, Bunker R K, Daly M J, Dzau V J, Goodman A, Koike G, Kren V, Kurtz T, Lernmark A
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown.
Nat Genet. 1995 Jan;9(1):63-9. doi: 10.1038/ng0195-63.
We report the construction of the first complete genetic linkage map of the laboratory rat. By testing 1171 simple sequence length polymorphisms (SSLPs), we have identified 432 markers that show polymorphisms between the SHR and BN rat strains and mapped them in a single (SHR x BN) F2 intercross. The loci define 21 large linkage groups corresponding to the 21 rat chromosomes, together with a pair of nearby markers on chromosome 9 that are not linked to the rest of the map. Because 99.5% of the markers fall into one of the 21 large linkage groups, the maps appear to cover the vast majority of the rat genome. The availability of the map should facilitate whole genome scans for genes underlying qualitative and quantitative traits relevant to mammalian physiology and pathobiology.
我们报告了实验室大鼠首张完整遗传连锁图谱的构建情况。通过检测1171个简单序列长度多态性（SSLPs），我们鉴定出432个在SHR和BN大鼠品系间表现出多态性的标记，并将它们定位在一个（SHR×BN）F2杂交群体中。这些位点定义了21个对应于21条大鼠染色体的大的连锁群，以及9号染色体上一对与图谱其他部分不连锁的相邻标记。由于99.5%的标记落入21个大的连锁群之一，这些图谱似乎覆盖了大鼠基因组的绝大部分。该图谱的可得性应有助于对与哺乳动物生理学和病理生物学相关的定性和定量性状的潜在基因进行全基因组扫描。
