Li S, Maruo T, Ladines-Llave C A, Samoto T, Kondo H, Mochizuki M
Department of Obstetrics and Gynecology, Kobe University School of Medicine, Japan.
Endocr J. 1994 Dec;41(6):693-701. doi: 10.1507/endocrj.41.693.
The cytologic localization and cellular levels of TGF alpha in the human ovary during follicular growth and regression were examined by avidin/biotin immunoperoxidase techniques with a monoclonal antibody to TGF alpha. In primordial follicles, only the oocyte showed intense immunostaining for TGF alpha, whereas the flattened pregranulosa cells were negative for the immunostaining. The earliest stage of follicular growth at which immunostaining for TGF alpha in granulosa cells and theca interna cells became apparent was the preantral stage. With the increase in the size of follicles, the intensity of TGF alpha immunostaining in the oocyte decreased, whereas the staining intensity of the granulosa and theca cells increased. The immunostaining for TGF alpha in granulosa and theca interna cells persisted in the corpus luteum, and further intensified during the midluteal phase. In the regressing corpus luteum, the immunostaining was present only in the peripheral theca lutein cells adjacent to the central scar tissue. The corpus albicans was negative for the immunostaining. Stromal cells exhibited weak staining for TGF alpha. In atretic follicles, the theca interna cells exhibited intense staining for TGF alpha without appreciable staining in the scattered granulosa cells. This is the first report to demonstrate that TGF alpha expression in the oocyte is maximal in primordial follicles, whereas TGF alpha expression in granulosa and theca cells increases with the progress of follicles and reaches its maximum in the lutein cells during the mid luteal phase.(ABSTRACT TRUNCATED AT 250 WORDS)
采用抗转化生长因子α（TGFα）单克隆抗体的抗生物素蛋白/生物素免疫过氧化物酶技术，检测了人卵巢卵泡生长和退化过程中TGFα的细胞定位及细胞水平。在原始卵泡中，仅卵母细胞显示出强烈的TGFα免疫染色，而扁平的颗粒前体细胞免疫染色呈阴性。颗粒细胞和卵泡内膜细胞中TGFα免疫染色开始出现的最早卵泡生长阶段是窦前期。随着卵泡大小的增加，卵母细胞中TGFα免疫染色强度降低，而颗粒细胞和卵泡膜细胞的染色强度增加。颗粒细胞和卵泡内膜细胞中的TGFα免疫染色在黄体中持续存在，并在黄体中期进一步增强。在退化的黄体中，免疫染色仅存在于与中央瘢痕组织相邻的周边黄体膜细胞中。白体免疫染色呈阴性。基质细胞对TGFα呈弱阳性染色。在闭锁卵泡中，卵泡内膜细胞对TGFα呈强烈染色，而散在的颗粒细胞中无明显染色。这是首次报道表明，卵母细胞中TGFα的表达在原始卵泡中最高，而颗粒细胞和卵泡膜细胞中TGFα的表达随卵泡发育而增加，并在黄体中期的黄体细胞中达到最大值。（摘要截短于250字）
