一段携带氨苄青霉素抗性基因的脱氧核糖核酸片段在大肠杆菌复制子之间的易位。
Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli.
作者信息
Bennett P M, Richmond M H
出版信息
J Bacteriol. 1976 Apr;126(1):1-6. doi: 10.1128/jb.126.1.1-6.1976.
Abstract
A number of well-characterized R plasmids (R1drd 19. K1.R100-1, R46, R55-1, R64-11, R388, and R751) will mobilize an integrated amp gene from the chromosome of Escherichia coli K-12. R391 will not act in this way. The process involves recombination, and in each case the mobilizing plasmid acquires an additional piece of deoxyribonucleic acid of molecular weight 4 X 10(6). Acquisition of this deoxyribonucleic acid may impair the transfer properties of the recombinant plasmid in some cases. The process will occur in a recA background.
摘要
一些特征明确的R质粒（R1drd 19、K1、R100 - 1、R46、R55 - 1、R64 - 11、R388和R751）能够从大肠杆菌K - 12的染色体上动员整合的氨苄青霉素基因。R391则不会以这种方式起作用。该过程涉及重组，并且在每种情况下，动员质粒都会获得一条分子量为4×10⁶的额外脱氧核糖核酸片段。在某些情况下，获得这种脱氧核糖核酸可能会损害重组质粒的转移特性。该过程将在recA背景下发生。
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