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巴拿马沙门氏菌中R因子聚集体内氨苄青霉素抗性决定子的易位

Translocation of an ampicillin resistance determinant within an R-factor aggregate in Salmonella panama.

作者信息

van Embden J D

出版信息

Antonie Van Leeuwenhoek. 1978;44(2):203-18. doi: 10.1007/BF00643223.

DOI:10.1007/BF00643223
PMID:582095
Abstract

The molecular properties of the plasmids of a natural isolate of Salmonella panama have been studied. This strain, Sp477, harbours 5 different plasmids: the conjugative plasmid pRI477TF (molecular weight 20 megadaltons), the two non-conjugative plasmids, pRI477A and pRI477S, coding for ampicillin and streptomycin plus sulfonamide resistance respectively (molecular weights of both 5.6 megadaltons) and two cryptic plasmids with molecular weights of 1.0 and 2.7, megadaltons respectively. After conjugal transfer to Escherichia coli the ampicillin resistance determinant was frequently found to be integrated into pRI477TF or pRI477S. The translocatable sequence on pRI477A, designated as Tn901, resembles the TnA sublcass transposon TnA(1).

摘要

对巴拿马沙门氏菌自然分离株的质粒分子特性进行了研究。该菌株Sp477含有5种不同的质粒:接合性质粒pRI477TF(分子量20兆道尔顿),两种非接合性质粒pRI477A和pRI477S,分别编码氨苄青霉素抗性和链霉素加磺胺抗性(分子量均为5.6兆道尔顿),以及两种隐蔽质粒,分子量分别为1.0和2.7兆道尔顿。在接合转移至大肠杆菌后,经常发现氨苄青霉素抗性决定簇整合到pRI477TF或pRI477S中。pRI477A上的可转移序列,命名为Tn901,类似于TnA亚类转座子TnA(1)。

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Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.根据脱氧核糖核酸在氯化铯中的浮力密度测定其碱基组成。
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Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli.细菌中的非染色体抗生素抗性。II. 从奇异变形杆菌和大肠杆菌中分离出的R因子的分子性质。
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