Fabret C, Quentin Y, Guiseppi A, Busuttil J, Haiech J, Denizot F
Laboratoire de Chimie Bactérienne, Marseille, France.
Microbiology (Reading). 1995 Feb;141 ( Pt 2):345-50. doi: 10.1099/13500872-141-2-345.
Increased productivity in DNA sequencing would not be valid without a straightforward detection and estimation of errors in finished sequences. The sequence of the surfactin operon from Bacillus subtilis was obtained by two different groups and by chance we were also working on the same chromosome region. Taking advantage of this situation we report in this paper, the number and nature of errors found in the overlapping part of the DNA sequences obtained by the three laboratories. The coincidence of some of the errors with compression in sequence ladders and with secondary DNA structures as well as the detection of frameshift errors using computer programs, are demonstrated. Finally we discuss the definition of a new sequencing strategy that might minimize both the error rate and the cost of sequencing.
如果不能直接检测和估计最终序列中的错误，DNA测序效率的提高就没有意义。枯草芽孢杆菌表面活性素操纵子的序列由两个不同的小组获得，碰巧我们也在研究同一染色体区域。利用这种情况，我们在本文中报告了三个实验室获得的DNA序列重叠部分中发现的错误数量和性质。文中展示了一些错误与序列梯中的压缩以及二级DNA结构的巧合，以及使用计算机程序检测移码错误的情况。最后，我们讨论了一种新的测序策略的定义，该策略可能会使错误率和测序成本降至最低。
