枯草芽孢杆菌中，srf操纵子的基因comS编码的开放阅读框的翻译对于遗传感受态的发展是必需的，但对于表面活性素的生物合成则不是必需的。
Translation of the open reading frame encoded by comS, a gene of the srf operon, is necessary for the development of genetic competence, but not surfactin biosynthesis, in Bacillus subtilis.
作者信息
D'Souza C, Nakano M M, Frisby D L, Zuber P
机构信息
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
出版信息
J Bacteriol. 1995 Jul;177(14):4144-8. doi: 10.1128/jb.177.14.4144-4148.1995.
Abstract
A small open reading frame, comS of the srf operon, is the site of mutations that impair competence development in Bacillus subtilis. comS open reading frame translation was required for competence, as was confirmed by the suppression of a comS amber mutation [comS(Am)] by the nonsense suppressor sup-3. comS(Am), when introduced into the srf operon, eliminated late competence gene expression but had no significant effect on surfactin production.
摘要
一个小的开放阅读框，即srf操纵子的comS，是枯草芽孢杆菌中影响感受态发育的突变位点。comS开放阅读框的翻译对于感受态是必需的，这一点通过无义抑制子sup-3对comS琥珀突变[comS(Am)]的抑制得到了证实。当将comS(Am)导入srf操纵子时，它消除了晚期感受态基因的表达，但对表面活性素的产生没有显著影响。
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