Okano K, Takada N, Kobayashi M, Maekawa H
Division of Radiation Control and Biology, University of Tokyo, Japan.
Insect Mol Biol. 1994 Nov;3(4):195-200. doi: 10.1111/j.1365-2583.1994.tb00167.x.
We have isolated a 1224 bp cDNA clone from a Bombyx mori embryonic cDNA library which contains sequences homologous to the kinesin-like protein gene, ncd, which is required for distribution of chromosomes at meiosis in Drosophila melanogaster females. This clone includes both a microtubule motor and the ATP-binding domains found in kinesin-like proteins. The motor domain is classified in the group of the BimC and cut7, which have a role in spindle formation during mitosis of Aspergillus nidulans and Schizosaccharomyces pombe, respectively. However, the location of the domain at the carboxy terminus is not common in this family, except for ncd and KAR3.
我们从家蚕胚胎cDNA文库中分离出一个1224 bp的cDNA克隆，该克隆含有与驱动蛋白样蛋白基因ncd同源的序列，ncd是黑腹果蝇雌性减数分裂时染色体分布所必需的。这个克隆包含一个微管马达和在驱动蛋白样蛋白中发现的ATP结合结构域。马达结构域属于BimC和cut7组，它们分别在构巢曲霉和粟酒裂殖酵母的有丝分裂纺锤体形成中发挥作用。然而，除了ncd和KAR3外，该结构域在羧基末端的位置在这个家族中并不常见。
