大红莓蚜（Amphorophora idaei，蚜亚科：大蚜族）核糖体间隔区长度变异性

Ribosomal spacer length variability in the large raspberry aphid, Amphorophora idaei (Aphidinae: macrosiphini).

作者信息

Birch A N, Fenton B, Malloch G, Jones A T, Phillips M S, Harrower B E, Woodford J A, Catley M A

机构信息

Scottish Crop Research Institute, Invergowrie, Dundee.

出版信息

Insect Mol Biol. 1994 Nov;3(4):239-45. doi: 10.1111/j.1365-2583.1994.tb00172.x.

DOI:10.1111/j.1365-2583.1994.tb00172.x

PMID:7704307

Abstract

The large raspberry aphid, Amphorophora idaei, has several biotypes described by their abilities to overcome plant resistance genes. Bioassays of field populations showed a strong shift towards A1 resistance-breaking biotypes since the 1960s. RFLP analysis of the rDNA cistron was used to study variation found within and between standard clones of three A. idaei biotypes and twenty-nine field populations collected over 3 years. Probing genomic DNA with the ribosomal DNA probe pBG 35 produced consistent differences in RFLPs between standard clones of biotypes. However, analysis of field populations gave more complex RFLP patterns that were not biotype-specific, unlike characteristic intergenic spacer (IGS) patterns reported for Schizaphis graminum biotypes. All but one sample collected from separate fields showed considerable genetic diversity within populations, attributed to alate migrations of parthenogenetic females in summer and males in autumn.

摘要

大型悬钩子蚜（Amphorophora idaei）有几种生物型，根据它们克服植物抗性基因的能力来描述。对田间种群的生物测定表明，自20世纪60年代以来，出现了向A1抗性破坏生物型的强烈转变。利用核糖体DNA顺反子的限制性片段长度多态性（RFLP）分析来研究三种大型悬钩子蚜生物型的标准克隆以及在3年时间里收集的29个田间种群内部和之间的变异。用核糖体DNA探针pBG 35探测基因组DNA，在生物型的标准克隆之间产生了一致的RFLP差异。然而，对田间种群的分析给出了更复杂的RFLP模式，这些模式不是生物型特异性的，这与报道的麦二叉蚜生物型的特征性基因间隔区（IGS）模式不同。从不同田块采集的样本中，除一个样本外，所有样本在种群内部都显示出相当大的遗传多样性，这归因于夏季孤雌生殖雌蚜和秋季雄蚜的有翅迁飞。