Simpson P J, Savage J R
MRC Radiobiology Unit, Chromosome Damage Group, Chilton, Didcot, UK.
Chromosome Res. 1995 Jan;3(1):69-72. doi: 10.1007/BF00711165.
In a recent series of papers we pointed out that any complex exchange (an interaction involving three or more breaks) can yield a wide range of aberration configurations. If these aberrations are detected with a single chromosome paint and total centromere probe, the majority will be scored as either 'simple' exchanges, apparently derived from two breaks, which we term pseudosimples, or as complexes which appear to have fewer breaks than were actually involved, which we term reduced complexes. In this study, metaphases from X-irradiated human fibroblasts were hybridized to two distinctly labelled composite probes (paints) for chromosomes 1 and 2. All centromeres were marked by differential 4',6-diamidino-2-phenylindole (DAPI) staining. Aberrations derived from exchanges involving both paints were characterized. With this method, we are able to identify some of the additional exchange events, so confirming the existence of these two classes of aberration. Ultimately we believe it can be used to estimate their frequency.
在最近的一系列论文中，我们指出任何复杂交换（涉及三个或更多断裂的相互作用）都能产生广泛的畸变构型。如果用单一染色体涂染探针和全着丝粒探针检测这些畸变，大多数将被记为“简单”交换，显然源自两个断裂，我们称之为假简单交换，或者记为看起来断裂数少于实际涉及的断裂数的复合体，我们称之为简化复合体。在本研究中，将经X射线照射的人成纤维细胞的中期染色体与针对1号和2号染色体的两种标记明显不同的复合探针（涂染探针）杂交。所有着丝粒都通过差示4',6-二脒基-2-苯基吲哚（DAPI）染色进行标记。对源自涉及两种涂染探针的交换的畸变进行了表征。通过这种方法，我们能够识别一些额外的交换事件，从而证实这两类畸变的存在。最终我们相信它可用于估计它们的频率。
