大肠杆菌中位于利福平（rif）基因座附近的两个核糖体蛋白顺反子的基因外抑制作用
Extragenic suppression of two ribosomal protein cistrons lying near the rif locus in Escherichia coli.
作者信息
Molholt B
出版信息
J Bacteriol. 1976 May;126(2):563-7. doi: 10.1128/jb.126.2.563-567.1976.
Abstract
The experiments reported here involve temperature-sensitive mutations in two of five cistrons encoding 50S ribosomal proteins that lie near the rif locus in Escherichia coli. I selected spontaneous TS+ mutants able to grow at elevated temperatures in which the TS+ event takes place outside this tract of cistrons near rif. Six distinct classes of extragenic suppressors were found, five of which have been mapped. Two of these suppressors lie near 64 min, a region known to be rich in cistrons ribosomal proteins (Dennis and Nomura, 1975). The remaining three extragenic suppressors were located near 16.5, 47, and 86 min.
摘要
本文报道的实验涉及大肠杆菌中五个编码50S核糖体蛋白的顺反子中的两个，这些顺反子位于rif位点附近，存在温度敏感突变。我筛选出了能够在高温下生长的自发TS+突变体，其中TS+事件发生在rif附近的这段顺反子区域之外。发现了六种不同类型的基因外抑制子，其中五种已被定位。其中两种抑制子位于64分钟附近，该区域已知富含核糖体蛋白顺反子（丹尼斯和野村，1975年）。其余三种基因外抑制子位于16.5、47和86分钟附近。
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