大肠杆菌中赭石突变型与错义抑制基因间的紧密连锁
Close linkage between ochre and missense suppressors in Escherichia coli.
作者信息
Orias E, Gartner T K, Lannan J E, Betlach M
出版信息
J Bacteriol. 1972 Mar;109(3):1125-33. doi: 10.1128/jb.109.3.1125-1133.1972.
Abstract
It was previously shown that the ochre suppressor mutation sup15B in Escherichia coli determines the accumulation of altered 30S ribosomal subunits and the presence of altered transfer ribonucleic acid (tRNA) capable of suppressing in vitro the UAG codon. This mutation has been mapped in the present study by means of conjugation and transduction experiments. After establishing the location of sup15B near argH, the following order was determined for the markers tested: metB-argH-(sup15B, supA36)-rif-thi. A comparison of location, growth rate, and suppressor pattern determined by sup15B and supM indicates the high probability that both suppressor mutations are identical. This study has also yielded a more precise location for the rifampin resistance gene. The most interesting finding is the very close (if not adjacent) location of the suppressor mutations sup15B and supA36, both of which determine tRNA alterations.
摘要
先前已表明，大肠杆菌中的赭石抑制突变体sup15B决定了改变的30S核糖体亚基的积累以及能够在体外抑制UAG密码子的改变的转移核糖核酸（tRNA）的存在。在本研究中，通过接合和转导实验对该突变进行了定位。在确定sup15B位于argH附近后，确定了所测试标记的以下顺序：metB-argH-（sup15B，supA36）-rif-thi。对由sup15B和supM确定的位置、生长速率和抑制模式进行比较表明，两种抑制突变很可能是相同的。本研究还为利福平抗性基因确定了更精确的位置。最有趣的发现是抑制突变体sup15B和supA36的位置非常接近（如果不是相邻的话），这两种突变都决定了tRNA的改变。
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本文引用的文献
1
Isolation of Pre-Adaptive Mutants in Bacteria by Sib Selection.通过同胞选择法分离细菌中的预适应突变体
Genetics. 1956 May;41(3):367-81. doi: 10.1093/genetics/41.3.367.
2
PLEIOTROPIC EFFECTS OF SUPPRESSORS OF A LAC-"OPERATOR NEGATIVE" MUTATION IN ESCHERICHIA COLI.大肠杆菌中乳糖操纵子“负”突变抑制子的多效性效应
Proc Natl Acad Sci U S A. 1965 Jan;53(1):62-8. doi: 10.1073/pnas.53.1.62.
3
GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI. IV. EFFECT OF A CHROMOSOMAL DELETION ON CHROMOSOME TRANSFER.大肠杆菌F'菌株介导的基因转移。IV. 染色体缺失对染色体转移的影响。
J Bacteriol. 1964 Aug;88(2):367-73. doi: 10.1128/jb.88.2.367-373.1964.
4
Suppressor gene alteration of protein primary structure.蛋白质一级结构的抑制基因改变。
Proc Natl Acad Sci U S A. 1963 Jul;50(1):9-16. doi: 10.1073/pnas.50.1.9.
5
Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles.大肠杆菌和痢疾志贺氏菌菌株间乳糖利用能力的转导及转导噬菌体颗粒的特性
Virology. 1960 Nov;12:348-90. doi: 10.1016/0042-6822(60)90161-6.
6
The kinetics of the mating process in Escherichia coli.大肠杆菌中交配过程的动力学
J Gen Microbiol. 1957 Feb;16(1):97-119. doi: 10.1099/00221287-16-1-97.
7
Amber mutants and chain termination in vitro.琥珀突变体与体外链终止
J Mol Biol. 1967 Oct 14;29(1):27-43. doi: 10.1016/0022-2836(67)90179-9.
8
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Genetics. 1966 Jul;54(1):61-76. doi: 10.1093/genetics/54.1.61.
9
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J Virol. 1968 Sep;2(9):905-14. doi: 10.1128/JVI.2.9.905-914.1968.
10
Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information.突变对链霉素抗性及突变基因信息翻译速率的影响。
J Bacteriol. 1966 Mar;91(3):1021-8. doi: 10.1128/jb.91.3.1021-1028.1966.
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