Wilson J M, Nobrega J N, Corrigall W A, Coen K M, Shannak K, Kish S J
Human Neurochemical Pathology Laboratory, Clarke Institute of Psychiatry, Toronto, Ont. Canada.
Brain Res. 1994 Dec 30;668(1-2):39-45. doi: 10.1016/0006-8993(94)90508-8.
The influence of cocaine on rat brain monoaminergic neurotransmitters (dopamine, serotonin, noradrenaline) and their metabolites, and on binding of [3H]WIN 35,428 and [3H]GBR 12,935 to the dopamine transporter was measured after 4 weeks of cocaine exposure. Cocaine (mean daily dose 9.25 +/- 0.48 mg/kg) was self-administered (responders) or passively received (yoked) during sessions which lasted for 1 h per day. As compared with the controls, mean dopamine and serotonin levels were significantly elevated (+ 107% and + 47%, respectively) in amygdala of responders, but not of yoked rats, sacrificed 1 h after the last cocaine session. Dopamine and metabolite levels were normal in all other brain areas examined, including striatum, nucleus accumbens and medial prefrontal cortex, at both 1 h and 4 weeks withdrawal from cocaine. [3H]WIN 35,428 and [3H]GBR 12,935 binding were unaltered after cocaine exposure. These data provide additional support for the involvement of the amygdala in the acquisition of drug seeking behavior associated with cocaine self-administration.
在给予可卡因4周后，测定了可卡因对大鼠脑单胺能神经递质（多巴胺、5-羟色胺、去甲肾上腺素）及其代谢产物的影响，以及对[3H]WIN 35,428和[3H]GBR 12,935与多巴胺转运体结合的影响。可卡因（平均日剂量9.25±0.48毫克/千克）通过自身给药（有反应者）或被动接受（配对者），每天给药1小时。与对照组相比，在最后一次可卡因给药1小时后处死的有反应者杏仁核中，多巴胺和5-羟色胺平均水平显著升高（分别升高107%和47%），而配对大鼠杏仁核中的这两种神经递质水平未升高。在停止给予可卡因1小时和4周时，在包括纹状体、伏隔核和内侧前额叶皮质在内的所有其他检测脑区中，多巴胺及其代谢产物水平均正常。给予可卡因后，[3H]WIN 35,428和[3H]GBR 12,935的结合未发生改变。这些数据为杏仁核参与与可卡因自身给药相关的觅药行为的获得提供了更多支持。
