铜绿假单胞菌在精液中对牛繁殖力影响的研究。
A study on the effect of Pseudomonas aeruginosa in semen on bovine fertility.
作者信息
Eaglesome M D, Garcia M M, Bielanski A B
机构信息
Department of Pathobiology, Agriculture Canada, Nepean, Ontario.
出版信息
Can J Vet Res. 1995 Jan;59(1):76-8.
Two experiments were done to demonstrate whether the presence of Pseudomonas aeruginosa in bovine semen could affect fertilization and/or early embryonic development. In the first experiment, superovulated heifers were inseminated with semen naturally contaminated with P. aeruginosa (ADRI 102) or clean semen and seven day-old embryos were collected nonsurgically. The endometrium of treated heifers appeared more sensitive to the flush procedures. In experiment 2, heifers were inseminated at synchronized estrus with semen experimentally contaminated with P. aeruginosa (ADRI 102) and processed in the same way as commercial semen with antibiotics (gentamicin, lincomycin, spectinomycin and tylosin) or processed without antibiotics added. Embryos were recovered at slaughter seven days later. In general, there was no significant reduction in fertility or development of embryos in vitro as a result of relatively high numbers of P. aeruginosa in bovine semen.
进行了两项实验，以证明牛精液中铜绿假单胞菌的存在是否会影响受精和/或早期胚胎发育。在第一个实验中，用自然感染铜绿假单胞菌（ADRI 102）的精液或清洁精液对超排的小母牛进行授精，并通过非手术方法收集7日龄胚胎。处理过的小母牛的子宫内膜对冲洗程序似乎更敏感。在实验2中，在同步发情时用实验性感染铜绿假单胞菌（ADRI 102）的精液对小母牛进行授精，并按照与添加抗生素（庆大霉素、林可霉素、壮观霉素和泰乐菌素）的商业精液相同的方式进行处理，或不添加抗生素进行处理。7天后在屠宰时回收胚胎。总体而言，由于牛精液中相对大量的铜绿假单胞菌，体外受精率或胚胎发育没有显著降低。
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