Gohel M D, Shum D K, Li M K
Department of Biochemistry, Faculty of Medicine, University of Hong Kong.
Clin Chim Acta. 1994 Nov;231(1):11-22. doi: 10.1016/0009-8981(94)90249-6.
The potential of whole urine of stone-formers and normal controls to precipitate crystals of calcium oxalate dihydrate from ions endogenous in urine was investigated by the method of rapid evaporation to 1250 mOsm/kg and subsequent freezing. Over 90% incidence in crystallization was achieved in the two groups. When the population density and size of these crystals were compared, it was found that 50% of urine samples from stone formers (30-50 years of age) were rich in crystal-nucleating and growth-inhibiting factors and the other 50% were not different from normal controls. These effects were observed when polyanionic macromolecules recovered from pooled urine samples of stone-formers or normal controls were dissolved in urine ultrafiltrate and similarly tested. That the urinary polyanions were the active modifiers in the test system was further corroborated by the reversal of crystallization activity of urine samples and the urinary polyanions after they had been through an acidification-reneutralization procedure. The urinary polyanionic macromolecules were found, after enzymatic and/or chemical treatment, to contain chondroitin sulphates, dermatan sulphates, heparan sulphates and glycoproteins.
通过快速蒸发至1250 mOsm/kg并随后冷冻的方法，研究了结石形成者和正常对照者的全尿从尿内源性离子中沉淀二水合草酸钙晶体的潜力。两组的结晶发生率均超过90%。当比较这些晶体的群体密度和大小时，发现结石形成者（30 - 50岁）50%的尿样富含晶体成核和生长抑制因子，另外50%与正常对照无差异。当将从结石形成者或正常对照者的混合尿样中回收的聚阴离子大分子溶解在尿超滤液中并进行类似测试时，观察到了这些效应。尿样和尿聚阴离子经过酸化 - 再中和程序后，其结晶活性发生逆转，进一步证实了尿聚阴离子是测试系统中的活性调节剂。经酶促和/或化学处理后，发现尿聚阴离子大分子含有硫酸软骨素、硫酸皮肤素、硫酸乙酰肝素和糖蛋白。
