Gohel M D, Shum D K, Li M K
Department of Applied Biology and Chemical Technology, Hongkong Polytechnic, Hunghom.
Urol Res. 1992;20(1):13-7. doi: 10.1007/BF00294328.
The nucleation-promoting and growth-inhibiting activities of urinary macromolecules on the crystallization of calcium oxalate endogenous in urine of stoneformers and normal controls were studied by freezing the ultrafiltrate and retenate fractions of concentrated whole urine (pH 5.3, 1,250 mosmol/kg). Among the normal controls, macromolecules nominally of 10-20 kDa showed nucleation-promoting and growth-inhibiting activities; the 5-10 kDa population was incapable of such effects but did cooperate with molecules greater than 10 kDa in enhancing the effect. In the case of stone-formers, molecules in the nominal ranges of 5-10 kDa and 10-20 kDa when considered separately were not active in the aspects studied but collectively could cooperate to produce pronounced effects. Application of the test to urine ultrafiltrate reconstituted with polyanionic macromolecules recovered from urine indicated that molecules from stoneformers were more powerful than those from normal controls in bringing about promotion of nucleation and inhibition of growth of crystals from urinary calcium oxalate.
通过冷冻浓缩全尿（pH 5.3，1250毫摩尔/千克）的超滤部分和保留部分，研究了尿大分子对结石形成者和正常对照者尿液中草酸钙结晶的成核促进和生长抑制活性。在正常对照者中，标称分子量为10 - 20 kDa的大分子表现出成核促进和生长抑制活性；5 - 10 kDa的群体没有这种作用，但确实与大于10 kDa的分子协同增强了这种作用。在结石形成者的情况下，单独考虑时，标称范围为5 - 10 kDa和10 - 20 kDa的分子在所研究的方面没有活性，但共同作用时可以协同产生显著效果。将该测试应用于用从尿液中回收的聚阴离子大分子重构的尿液超滤物表明，结石形成者的分子在促进草酸钙尿结晶的成核和抑制生长方面比正常对照者的分子更有效。
