Conlon K, Osborne J, Morimoto C, Ortaldo J R, Young H A
Laboratory of Experimental Immunology, National Cancer Institute, Frederick, MD 21702-1201, USA.
Eur J Immunol. 1995 Mar;25(3):644-8. doi: 10.1002/eji.1830250303.
Flow cytometric analysis of human peripheral blood T lymphocytes demonstrated that the majority of the CD4+ cells were CD29+ or CD45RO+ "mature" cells while the CD8+ cells were primarily CD45RA+ "native" cells. After an initial separation into CD4+ and CD8+ cells and a secondary separation into CD45 subsets, lymphokine secretion was assessed after phorbol 12-myristate 13-acetate and ionomycin or fixed anti-CD3 stimulation. Within the respective CD45 subsets, CD4+ cells produced more interleukin (IL)-2, IL-4, and IL-6; but the CD8+ cells secreted more interferon-gamma and granulocyte/macrophage-colony-stimulating factor. Tumor necrosis factor-alpha secretion was similar in the matched CD45 subsets. Northern analysis revealed a parallel pattern of lymphokine mRNA expression in the four lymphocyte subsets. These results suggest that human CD8+ peripheral blood lymphocytes have a significant capacity to secrete lymphokines, and that the low lymphokine production observed in unseparated CD8+ cells reflects the higher percentage of less functional CD45RA+ cells.
对人外周血T淋巴细胞进行的流式细胞术分析表明，大多数CD4+细胞是CD29+或CD45RO+“成熟”细胞，而CD8+细胞主要是CD45RA+“天然”细胞。在初步分离为CD4+和CD8+细胞并进一步分离为CD45亚群后，用佛波醇12-肉豆蔻酸酯13-乙酸酯和离子霉素或固定抗CD3刺激后评估淋巴因子分泌。在各自的CD45亚群中，CD4+细胞产生更多的白细胞介素（IL）-2、IL-4和IL-6；但CD8+细胞分泌更多的干扰素-γ和粒细胞/巨噬细胞集落刺激因子。肿瘤坏死因子-α在匹配的CD45亚群中的分泌相似。Northern分析揭示了四个淋巴细胞亚群中淋巴因子mRNA表达的平行模式。这些结果表明，人CD8+外周血淋巴细胞具有显著的分泌淋巴因子的能力，并且在未分离的CD8+细胞中观察到的低淋巴因子产生反映了功能较差的CD45RA+细胞的较高百分比。
