Conlon K, Lloyd A, Chattopadhyay U, Lukacs N, Kunkel S, Schall T, Taub D, Morimoto C, Osborne J, Oppenheim J
Laboratory of Experimental Immunology, NCI-FCRDC, MD 21702-1201, USA.
Eur J Immunol. 1995 Mar;25(3):751-6. doi: 10.1002/eji.1830250319.
The chemokines macrophage inflammatory protein 1 alpha (MIP 1 alpha), interleukin-8 (IL-8) and RANTES are potent regulators of leukocyte trafficking. Examination of chemokine secretion by human peripheral blood lymphocytes after stimulation with anti-CD3 or phorbol 12, 13 myristate acetate and ionomycin showed CD8+ cells were the dominant source of MIP 1 alpha and RANTES. Although production of MIP 1 alpha and IL-8 were similar in pharmacologically stimulated CD4+ CD45RA+, CD4+ CD45RO+, and CD8+ CD45RA+ cells, the largest amounts of MIP 1 alpha and RANTES were secreted by CD8+ CD45RO+ lymphocytes. A parallel pattern of prolonged chemokine mRNA expression for at least 18 h after activation was observed in the T cells subsets. These results confirm that human T lymphocytes have a unique capacity for secretion of these three chemokines. In addition, CD8+ cells have an unrecognized role in recruiting cells to sites of inflammation, and adult human CD45RA+ cells have a physiologically significant secretory capacity.
趋化因子巨噬细胞炎性蛋白1α（MIP 1α）、白细胞介素-8（IL-8）和调节激活正常T细胞表达和分泌的因子（RANTES）是白细胞迁移的有效调节因子。在用抗CD3或佛波醇12、13十四酸酯乙酸盐和离子霉素刺激后人外周血淋巴细胞趋化因子分泌的检测显示，CD8 +细胞是MIP 1α和RANTES的主要来源。虽然在药理学刺激的CD4 + CD45RA +、CD4 + CD45RO +和CD8 + CD45RA +细胞中MIP 1α和IL-8的产生相似，但CD8 + CD45RO +淋巴细胞分泌的MIP 1α和RANTES量最大。在T细胞亚群中观察到激活后至少18小时趋化因子mRNA表达延长的平行模式。这些结果证实，人T淋巴细胞具有分泌这三种趋化因子的独特能力。此外，CD8 +细胞在将细胞募集到炎症部位方面具有未被认识的作用，而成人CD45RA +细胞具有生理上显著的分泌能力。
